LabCanvas Image Analysis measures microscope images, western blots and gels in the browser, always on the raw pixel values. It opens TIFF files from the microscope, including stacks, 16- and 32-bit images and ImageJ, OME-TIFF and BigTIFF files, which keep their scale.
Tools are grouped by task: processing (background, filters, contrast, drift correction, stain separation), measuring (ROIs, intensity profiles, colocalisation), counting (threshold and watershed, a pixel classifier trained with brush strokes, a cell counter, batch processing) and blots (lanes, bands, a molecular-weight ladder and normalisation). Guided assays cover nuclear translocation, spots per cell, wound healing, FRAP, calcium, tracking and branching. Results go to Graphs with a methods sentence.
Read more: Image Analysis features · How to use Image Analysis · Guide: image analysis for microscopy and western blots · How the measurements are validated.