Graphical abstract template · Multipanel figures · Worked examples
Four-panel figure: cell viability and cytotoxicity
A cell-based assay figure: the treatment workflow on a 96-well plate, live/dead fluorescence images of control and treated cells, dose–response curves with fitted IC₅₀ values for two cell lines, and viability at one dose with every replicate and a one-way ANOVA against the control.
Updated 2 cited papers147 editable layers
At a glance
- Category
- Multipanel figures
- Method
- Worked examples
- Product
- A 2 × 2 figure (a–d) for an in vitro efficacy or toxicity study
- Key result
- Example data from logistic curves with noise; images drawn
- Figure
- 657.64 × 358.35 px (16:9), 147 editable layers, 6 generated objects
Step by step
- a · WorkflowSeed, treat and read a 96-well plate: times and conditions as badges
- b · ImagesLive (green), nuclei (blue) and dead (red) cells, untreated and treated: drawn stand-ins with 100 µm scale bars
- c · Dose–responseMean ± SEM of triplicates with a four-parameter logistic fit for each cell line
- d · One doseEvery replicate on the bars, one-way ANOVA with Dunnett’s test against the control
Strengths
- Shows every data point (Weissgerber et al. 2015)
- Statistics computed in the graph and updated with the data
Limitations
- Example values only
Uses
- Drug, nanoparticle and material toxicity
- Screening hits
Papers this template follows
- Weissgerber, Milic, Winham & Garovic, “Beyond bar and line graphs: time for a new data presentation paradigm”, PLoS Biol. 2015. doi:10.1371/journal.pbio.1002128review
- Rougier, Droettboom & Bourne, “Ten simple rules for better figures”, PLoS Comput. Biol. 2014. doi:10.1371/journal.pcbi.1003833review
Example data generated for this template; micrographs are drawn stand-ins. Replace them with your own.
How templates are written and checked: editorial guidelines.
Questions
What does the “Four-panel figure: cell viability and cytotoxicity” template show?
A cell-based assay figure: the treatment workflow on a 96-well plate, live/dead fluorescence images of control and treated cells, dose–response curves with fitted IC₅₀ values for two cell lines, and viability at one dose with every replicate and a one-way ANOVA against the control.
What are the steps shown in “Four-panel figure: cell viability and cytotoxicity”?
1. a · Workflow: Seed, treat and read a 96-well plate: times and conditions as badges. 2. b · Images: Live (green), nuclei (blue) and dead (red) cells, untreated and treated: drawn stand-ins with 100 µm scale bars. 3. c · Dose–response: Mean ± SEM of triplicates with a four-parameter logistic fit for each cell line. 4. d · One dose: Every replicate on the bars, one-way ANOVA with Dunnett’s test against the control.
Which papers is “Four-panel figure: cell viability and cytotoxicity” based on?
Weissgerber, Milic, Winham & Garovic, “Beyond bar and line graphs: time for a new data presentation paradigm”, PLoS Biol. 2015 (doi:10.1371/journal.pbio.1002128); Rougier, Droettboom & Bourne, “Ten simple rules for better figures”, PLoS Comput. Biol. 2014 (doi:10.1371/journal.pcbi.1003833).
Can I edit the “Four-panel figure: cell viability and cytotoxicity” template?
Yes. Open it in the LabCanvas editor: all 147 layers are editable, including 6 generated objects (structures, plots, equipment) with their own settings. Change the text, colours and layout, then export PNG, TIFF, SVG or PDF.
Do I need an account to use this template?
Not to view it here. To open and edit it, sign in to LabCanvas or create a free account (the free plan keeps up to 3 designs). Downloading the figure is part of LabCanvas Pro (US$19 a month or US$199 a year).
